Samiya Siddiqui

8 Questions 12 Answers 0 Followers

Questions related from Samiya Siddiqui

Can someone please suggest me a protocol for Electrophoretic mobility shift assay (EMSA)?

I have to check that whether my purified protein interacts with DNA for which i need to perform an EMSA. I am unable to design this experiment properly so need some tips and suggestions. Thankyou...

05 December 2023 3,820 0 View

Can someone explain that why my 0.7% agarose gel takes 2 hrs to run and also some suggestions on cloning for bacterial two hybrid experiment?

I am doing cloning of a big bacterial insert (3705bp) into a vectors of varying sizes ranging from 3017bp to 3469bp for my bacterial two hybrid experiment. Among other problems with my cloning I...

16 November 2023 7,070 4 View

Which biochemical identification tests can be performed for the identification of lactobacilli or any lactic acid bacteria?

Can anyone help me that which tests can i use for identification of lactic acid bacteria. Can mannitol salt agar test be used for lactobacilli or MSA is only used as a selective media for growth...

04 January 2019 3,954 10 View

What is the effect of Resistant Starch feed on serum insulin level?

Resistant starch containing feed has been given to rats for invivo modelling.What should be the proposed effect of this feed on serum insulin level?

29 July 2018 3,643 4 View

What is the most feasible method of invitro digestion which mimicks the entire gastrointestinal digestion of Resistant Starch ?

Gastrointestinal digestion includes the entire digestive pattern of any substance starting from mouth, stomach till the small intestine. Is there any protocol which helps to determine the in-vitro...

24 June 2018 1,161 4 View

How to make 0.6mM Iodine solution in 10% DMSO?

Should i use potassium iodide or iodine crystals or both of them?

10 May 2018 7,039 3 View

Can anyone tell me that which is the best protocol for iodine binding ability of Resistant starches ?

i followed two different protocols but my resistant starch sample does not dissolve easily in 90%DMSO and forms swollen granules due to which doesn't bind with iodine and shows lesser binding as...

07 April 2018 3,550 3 View

What is the reason for decrease in percent transmittance %T of starch samples with refrigerated storage?

Can anyone explain that what is the reason behind decrease in percent transmittance %T of starch samples when subjected to refrigerated storage for a period of 5 days. According to articles with...

28 March 2018 2,881 12 View