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Questions related from Samiya Siddiqui
Hi everyone, I'm currently working on a ChIP-seq experiment and encountering an issue I hope someone can help me troubleshoot. My experimental workflow overview is as follows: • Cloned a...
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Hi everyone, I am currently working on expressing a His-tagged protein of interest using a pHERD20T vector. I have successfully cloned my gene into the vector and am now attempting to...
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Hi everyone, I have a DNA sequence (Phage DNA) that I want to digest using restriction enzymes to generate fragments of different lengths. However, I suspect that there might be modifications in...
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Hello, I am trying to fix Pseudomonas aeruginosa PAO1 cells with 4% formaldehyde for 20-30 minutes (on ice and in the dark) for fluorescent microscopy. I am attempting to stain the DNA and outer...
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I have to check that whether my purified protein interacts with DNA for which i need to perform an EMSA. I am unable to design this experiment properly so need some tips and suggestions. Thankyou...
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I am doing cloning of a big bacterial insert (3705bp) into a vectors of varying sizes ranging from 3017bp to 3469bp for my bacterial two hybrid experiment. Among other problems with my cloning I...
16 November 2023 7,138 4 View
Can anyone help me that which tests can i use for identification of lactic acid bacteria. Can mannitol salt agar test be used for lactobacilli or MSA is only used as a selective media for growth...
04 January 2019 3,988 10 View
Resistant starch containing feed has been given to rats for invivo modelling.What should be the proposed effect of this feed on serum insulin level?
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Gastrointestinal digestion includes the entire digestive pattern of any substance starting from mouth, stomach till the small intestine. Is there any protocol which helps to determine the in-vitro...
24 June 2018 1,220 4 View
Should i use potassium iodide or iodine crystals or both of them?
10 May 2018 7,117 3 View
i followed two different protocols but my resistant starch sample does not dissolve easily in 90%DMSO and forms swollen granules due to which doesn't bind with iodine and shows lesser binding as...
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Can anyone explain that what is the reason behind decrease in percent transmittance %T of starch samples when subjected to refrigerated storage for a period of 5 days. According to articles with...
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