Samiya Siddiqui

12 Questions 22 Answers 0 Followers

Questions related from Samiya Siddiqui

Troubleshooting ChIP-seq: No Protein band in Elution – Expression or Immunoprecipitation (IP) Failure?

Hi everyone, I'm currently working on a ChIP-seq experiment and encountering an issue I hope someone can help me troubleshoot. My experimental workflow overview is as follows: • Cloned a...

12 August 2025 4,436 1 View

How Can I Resolve Issues with Electroporation of PAO1K Using pHERD20T Vector?

Hi everyone, I am currently working on expressing a His-tagged protein of interest using a pHERD20T vector. I have successfully cloned my gene into the vector and am now attempting to...

26 March 2025 2,960 2 View

How to Identify DNA Modifications and Select Suitable Restriction Enzymes for Digestion?

Hi everyone, I have a DNA sequence (Phage DNA) that I want to digest using restriction enzymes to generate fragments of different lengths. However, I suspect that there might be modifications in...

02 October 2024 531 16 View

Does Anyone Have a Reliable Protocol for Fixing PAO1 Cells for fluorescent microscopy?

Hello, I am trying to fix Pseudomonas aeruginosa PAO1 cells with 4% formaldehyde for 20-30 minutes (on ice and in the dark) for fluorescent microscopy. I am attempting to stain the DNA and outer...

19 July 2024 7,850 3 View

Can someone please suggest me a protocol for Electrophoretic mobility shift assay (EMSA)?

I have to check that whether my purified protein interacts with DNA for which i need to perform an EMSA. I am unable to design this experiment properly so need some tips and suggestions. Thankyou...

05 December 2023 3,906 0 View

Can someone explain that why my 0.7% agarose gel takes 2 hrs to run and also some suggestions on cloning for bacterial two hybrid experiment?

I am doing cloning of a big bacterial insert (3705bp) into a vectors of varying sizes ranging from 3017bp to 3469bp for my bacterial two hybrid experiment. Among other problems with my cloning I...

16 November 2023 7,152 4 View

Which biochemical identification tests can be performed for the identification of lactobacilli or any lactic acid bacteria?

Can anyone help me that which tests can i use for identification of lactic acid bacteria. Can mannitol salt agar test be used for lactobacilli or MSA is only used as a selective media for growth...

04 January 2019 3,995 10 View

What is the effect of Resistant Starch feed on serum insulin level?

Resistant starch containing feed has been given to rats for invivo modelling.What should be the proposed effect of this feed on serum insulin level?

29 July 2018 3,702 4 View

What is the most feasible method of invitro digestion which mimicks the entire gastrointestinal digestion of Resistant Starch ?

Gastrointestinal digestion includes the entire digestive pattern of any substance starting from mouth, stomach till the small intestine. Is there any protocol which helps to determine the in-vitro...

24 June 2018 1,229 4 View

How to make 0.6mM Iodine solution in 10% DMSO?

Should i use potassium iodide or iodine crystals or both of them?

10 May 2018 7,126 3 View

Can anyone tell me that which is the best protocol for iodine binding ability of Resistant starches ?

i followed two different protocols but my resistant starch sample does not dissolve easily in 90%DMSO and forms swollen granules due to which doesn't bind with iodine and shows lesser binding as...

07 April 2018 3,620 3 View

What is the reason for decrease in percent transmittance %T of starch samples with refrigerated storage?

Can anyone explain that what is the reason behind decrease in percent transmittance %T of starch samples when subjected to refrigerated storage for a period of 5 days. According to articles with...

28 March 2018 2,932 12 View