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Hello everyone, I have beginning work in a new lab where I never use the cryostat (but I did it a ton of times in my previous lab). I cut mouse visual cortex at -20 and -18 with sections of 20 um....
25 February 2020 4,533 3 View
I am trying to make a ChIP, following by qPCR not by ChIPseq, from 10.000 facsed nuclei as starting material. Could someone suggest to me a protocol that could work with such a low amount of...
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I have performed a ChIP experiment and I have shared DNA ( 200-500 bp) and I checked if I have well immunoprecipitated the transcription factor of interest by Western Blot (and I succed in this)....
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