How one can get copyrights for previously published diagrams?

Usually review articles contain two or more diagrams from previously published research paper. What are the procedures to get copy rights from a journal to reproduce one or two diagrams in a...

25 January 2020 8,121 3 View

How to use fatigue tool for composite in a Ansys workbench?

Currently doing final year project in a leaf spring fatigue test in Ansys workbench 16.0. we have done fatigue test for steel, but error came for composite while testing we don't know how to...

07 March 2019 3,100 2 View

What is the procedure to calculate biomass efficiency?

I have carried out pyrolysis of biomass. Now I am in need of calculating the biomass efficiency (in terms of mass and energy conversion). Kindly help me with a formula.

21 July 2018 457 4 View

Does anyone have experienced the white band pattern in western blot technique for phosphorylated form of AKT protein expression?

03 March 2017 1,441 4 View

How important is considering the exon-exon junction for primer design in order to perform RT-real time PCR by SYBR-green?

How important is considering the exon-exon junction for primer design in order to perform RT-real time PCR by SYBR-green?

07 February 2017 2,506 2 View

How to do Two-way ANOVA in SPSS for four groups and comparing between two different time points within the groups and between the groups?

Hello to everyone I am working in the field of stress physiology, I am comparing different parameters between Male animals Vs Female animals and within Male and female animals, each group contains...

12 October 2016 4,924 2 View

How an atom probe Tomography is advanced from conventional TEM and SEM?

07 October 2016 6,375 5 View

How to select the appropriate statistical method for Questionnaire based survey study?

28 June 2016 9,779 3 View

Can someone provide clarification about the relation between capacitance and zeta potential?

I am working on graphene nanocomposite electrochemistry. I want know about relation between capacitance and zeta potential?

22 February 2015 3,097 4 View

What is the maximum ramp rate for power reversal in VSC-HVDC systems?

29 November 2014 597 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View