I have obtained the source sequence of many proline metabolic genes from NCBI (such as p5cs1, p5cs2, p5cr, oat, pdh1, pdh2, etc.). The title of the genes varies with partial cd, complete cd, mRNA, and sometimes only gene. What does this signify? In the case of expression study, do we have to design a primer based on cd seq or is it fine if we use the whole gene seq?
Would a cd sequence be appropriate for a primer design for a real time assay? I was thinking that maybe I should choose a cd sequence or go for a gene and then use the exons of that gene.
i would like to correct my question ...I mean PROLINE METABOLIC GENES...like p5cs1 , p5cs2, p5cr, oat, pdh 1 and pdh2....
i want to design primers for expression study of proline metabolic genes using real time assay...so for this purpose should I select cd sequence or go for gene and then use exons of that gene.
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