Can someone help me with a mice cardiomyocytes culture protocol?

12 September 2013 1 7K Report

I am trying to establish cardiac cell or cardiomyocytes isolation and culture from a mouse heart. I need a convenient protocol, if somebody can help me out. As far as I know the heart has to be isolated fresh and proceeded. Can we try storing heart tissue at -80?

Raise Ahmad

Thanks firdos for the information..............actually we want to check some protein exopression in cardiac cells..............will that be possible in adult mouse heart........can we stimulate and keep them at least for 48 hours.....

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