Do you recommend an effective anti-OCT4 Ab for IHC?

I'm staining some brains with several stemness markers but the OCT4 antibody that I have been using is not working. I would really appreciate your recommendations! Thank you!

25 January 2021 3,169 3 View

Why does bromophenol blue band seem to split up during this SDS PAGE run?

06 January 2021 7,788 4 View

Why do I keep obtaining the same secondary PCR band along with the specific product band?

Hello everyone! I need to amplify a specific DNA fragment in order to do Sanger sequencing for the detection of a point mutation in human patients. The fragment is in an exon of my gene of...

25 December 2020 1,805 3 View

How is possible that only a small percentage of bromophenol blue started the run in agarose electrophoresis ?

I used an 1,5% agarose gel for a second time and I loaded in the wells that I never used 15 microlitres of sample (constituted of 3 microlitres of bromophenol blue 6X, 6 microlitres of sample and...

07 December 2020 1,373 3 View

Is it correct to expect the same quantitative result between SDS-PAGE and BN-PAGE analysis performed on the same sample?

Please, can anyone please help me with this issue? By BN-PAGE and western blot on DDM-solubilized mitochondria, isolated from cultured cells, I found that in treated cells the amount of CV...

05 December 2020 7,696 2 View

How can I calculate degrees of freedom and write F for repeated measure ANOVA?

How can I calculate and report degrees of freedom for repeated mesure ANOVA? I have 48 observations N=48 and 2 factors of 3(P) and 8(LA) levels. I calculate degrees of freedom as follows: dF P =...

19 November 2020 5,682 5 View

What is the best pain killer for mice?

Good morning, I need to implant chronic electrodes in mice muscles. What is the best injectable painkiller to use during and after the surgery? and what the recommended dose for mice? And for how...

01 November 2020 8,477 1 View

Can the ramp rate of a Thermal Cycler machine influence the PCR result?

Hello, I am running PCR for genotyping. I didn't have problem till this Summer, when I couldn't see any band on the gel. I checked primers, Taq, etc. I bought new stocks etc. But still I can't...

16 September 2020 7,660 5 View

Does anyone know trait-based ecology approaches to define spatial boundaries or limits for decision making?

Dear colleagues. Does anyone know of any work in which the criteria of functional traits of plants related to hydrological ecosystem services (e.g., hydrological regulation, drought mitigation or...

03 June 2020 9,036 3 View

How do I determine sample size for each strata in survey?

Hello, I'm currently trying to perform a supervised classification of land cover for remote sensing images. In order to do that, I must create a training imput where I classify a fraction of the...

01 May 2020 7,696 3 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

How to improve and implement WBAN MAC and Radio modules under Castalia 3.2 ?

Hello researchers, Hello Castalia3.2 researchers I am a beginner with Castalia, I work on optimization of RFID systems used in wireless body networks. I simulated a WBAN with 10 nodes and one...

28 February 2021 400 1 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

I have need of XRD graph analysis software for phase identification. Can you please provide links for software tools which can be downloaded?

i am trying to download x'pert high score software, but can't download it. please do suggest software for phase analysis

24 February 2021 5,687 4 View