Can someone help me determine the sizes of DNA sequences in my PCR and Saccharomyces cerevisiae genome using the below document as guidance?

More Stefan Bishop's questions See All

My supposition or question: Is the 600 tetrahedron cell {3,3,5} polytope a four dimensional quasicrystal? Or is it more like a crystal twin?

My supposition or question: Is the 600 tetrahedron cell {3,3,5} polytope a four dimensional quasicrystal? I do think so, because its circumsphere is described by a radius of exactly the golden...

16 July 2024 4,653 0 View

How to find the next transformer / LLM?

Imagine having a rough idea for an alternative building block of large language models other the well-known transformers. In your head, the idea appears reasonable and overcome some perceived...

28 May 2024 2,723 3 View

Are there non-neuronal (linear) object perception algorithms?

Neuronal networks and deep learning algorithms are not appropiate for high safety levels applications (e.g. ADAS/AD). The possible test coverage is to low. Are there any other approches?

18 March 2024 6,836 6 View

Can anyone identify the Genus and Family of this transparent cave-dwelling snail?

Last weekend I took a photo of this cave-dwelling snail in a northern peruvian cave (1200 metres above sea level). Could you please help me to identify the Genus and Family? Thank you very much.

02 March 2024 5,416 5 View

Why do eastern European nations seem to have a significantly lower all-cause mortality rate than the UK, Canada, and the U.S.?

From what I can tell by searching the "Your World in Data" website, eastern Europe seems to have an expected lower than average all-cause mortality rate than the western first world nations, who...

04 January 2024 7,066 0 View

Can an article for a systematic review be excluded because it is unattainable due to cost?

Would providing an explanation due to a high cost of an article and unavailability in the library be a good reason for exclusion criteria when working on a systematic review?

19 December 2023 8,926 3 View

White streaks on mouse liver - any ideas?

After opening up the mice, sometimes I notice white streaks on the liver as shown in the picture. The mice are usually 6-8 weeks old, fed regular chow and water. No treatment was applied and we...

13 December 2023 3,605 0 View

Can anyone identify the Genus and Family of this cave-dwelling harvestman?

Last weekend I took a photo of this cave-dwelling harvestman in a northern peruvian cave (3000 metres above sea level). Could you please help me to identify the Genus and Family? Thank you very...

10 December 2023 3,747 0 View

What is the first 4D movie to use the principles of linear perspective to illustrate the relationships in a four-dimensional coordinate system?

15 November 2023 1,968 0 View

How many people have read the book"Four-Dimensional Descriptive Geometry " by C. Ernesto S. Lindgren and Steve M. Slaby?

How many people have read the book Four-Dimensional Descriptive Geometry by C. Ernesto S. Lindgren and Steve M. Slaby?

14 November 2023 6,864 2 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Is it true that the vacuum bomb is about to arrive?

We assume this to be true. We also assume that the vacuum bomb is the latest version of explosives with an explosive power of a few to ten kg of TNT equivalent. It has the unique characteristic of...

04 August 2024 4,534 1 View

Identify the eight key processes of excellent supply chain management and discuss how each of these processes impacts the end customer?

Read the journal article by Douglas M. Lambert, “The Eight Essential Supply Chain Management Processes,” Supply Chain Management Review, Vol. 8, No. 6 (2004), pp. 18-26

04 August 2024 9,919 4 View

Abhijeet Singh

for PCR fragment do gel electrophoresis
for genome search in public databases

Describe briefly what you want help with, don't give job to others to read and answer for you. Its you who is seeking answer.

Prarthna Saraswat

simply perform a blast search for your PCR DNA fragments with that of S.cerevisiae genome database, hits will show you genes involved

Prarthna Saraswat Can you please tell us how one can blast a PCR DNA fragment/amplicon?