I have an undiluted drug in powder form. I need to give it to my 20 gram mouse at a dose of 10 mg/Kg. The drug is docetaxel and its MW is 807.87922.
The drug product sheet says that it is soluble in 5 mg/ml DMSO and then can be diluted to 0.1 mg/ml in a 1:10 solution of DMSO;PBS. How can I do these two dilutions so that I can perform an i.p. injection into a mouse of up to 200 ul? Can you do i.p. injections of more than 200 ul?
The ip dose volume for mice is generally accepted as 10 ml/kg. Your drug dose of docetaxel is 10 mg/kg, therefore the dose concentration is 1 mg/ml. You can try and work out the minimum of percentage of DMSO is able to dissolve as 1 mg/ml of docetaxel. That % DMSO is also to be used as your vehicle control. Good luck to your experiments!
Prepare a 5 mg/mL solution of tour drug in DMSO. Administer 40 microlitre of this solution to the 20 g mouse.
Note: This will deliver 200 microgram of the drug into the mouse, equivalent to the dose of 10 mg/kg. It will also ensure that the dose volume is within the accepted 10 mL/kg level for mice.
In our lab we are using taxol as a positive control for human NSCLC at 20 mg/kg (see the attached article).
If you want to have tips about the formulation, you should contact Dr. Nathalie Wauthoz (on my behalf) who is registered on RG.
Best regards
Robert
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