For example, if I have excessive activity of a protein, normal activity and null activity, should I code them as 2, 1 and 0 then enter them as an allele frequency data for each population?
See if this helps
https://www.eeb.ucla.edu/Faculty/Barber/Web%20Protocols/Arlequin.pdf
Check the manual also
http://cmpg.unibe.ch/software/arlequin35/man/Arlequin35.pdf
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