Hi!

I had good results after using this one:

"This protocol details the steps found to be necessary when inducing protein expression in a plasmid containing an L-rhamnose or Larabinose inducible promoters. The protocol conditions were determined through trial and error in E. coli strains BL21(DE3) and Top10, using FimH-sfGFP fusion constructs made by the Exeter iGEM team (2017). 1. Inoculate 50 ml of LB with a colony of the pertinent bacteria. 2. Grow culture to an optical density of 0.6. 3. Induce using the appropriate sugar. The culture should be made up to a 2% concentration of the sugar. Aliquots of the sugar in solution can be made up and filtered (preferable to autoclaving due to caramelisation) in advance. The concentration of the aliquot seems to be limited to 50%, above which the sugar comes out of solution very easily. 4. Allow to grow “overnight”. According to various sources the protein of interest can begin to be expressed after ~5hours, but an overnight culture will ensure synthesis. This should be done at 37˚C, 220RPM. "

source: http://2017.igem.org/wiki/images/c/c9/T--Exeter--Induction_protocol.pdf

Hi there,

Depending on the target, arabinose concentration can be optimized from as little as 0.001% up to 1%.

I think the induction temperature may need to be experimentally optimized for some proteins.