Can someone advise on whether to express protein in 1x250 ml or 5x50 ml?

Morteza Zahedi @Morteza_Zahedi2

09 September 2013 1 5K Report

I want to express my protein (in E. coli) in 250 ml of culture medium (LB Broth).

Is it better to culture my bacteria in one bottle (1 x 250 ml) or aliquot them in 5 separated bottles (5 x 50 ml)?

What is your reason(s)?

Luigi Martino

Hi,

The ratio between the size of the flask used for the growth and the volume of media in the flask is an important parameter that affects the oxygenation of the cells. Usually, I keep this ratio around 4 or even above 4. This means that I would grow 250 mL of bacteria in a 1L flask. Hope this will help.

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