Why battery always retain the same value of power?

Dear all, I have been testing a model that is in the Matlab library (https://es.mathworks.com/help/physmod/sps/ug/simplified-model-of-a-small-scale-micro-grid.html), this is named "power...

10 November 2020 2,117 3 View

How does methylene blue fluoresce under dark UV?

Will it show as blue color under dark UV light (say 390 nm to 400 nm range) ? Does it degrade over time under long exposure of UV light (i.e. losing its fluorescein characters) ? What...

23 September 2020 9,752 2 View

How to avoid blockages of centrifuge filters when filtering polydisperse liposome samples for separation of free hydrophobic drug?

I'm using ultrafiltration for the separation of free curcumin from liposomes. The tubes are 1ml 0.45um cellulose acetate filter tubes (Corning Spin-X) and the centrifuge I'm using is the Thermo...

01 September 2020 8,875 2 View

What benchtop high pressure homogeniser for downsizing liposomes would you choose and why ?

Emphasis on: Minimising drug and lipid loss Minimising number of passes Achieving unimodal distribution Maintaining high encapsulation efficiencies Ease of use and maintenance

24 November 2019 1,946 2 View

How to quantify drug uptake of each cell line of co-cultured cells?

22 October 2019 3,153 3 View

Measuring presence and immersion: Within or between groups?

Hi, I am currently working on a project where I am looking to measure variables such as sense of presence, immersion, and realism using a self-report questionnaire. Participants are to be divided...

11 July 2019 943 1 View

Does anyone have a protocol of loading liposomes with doxorubicin?

I'm intending to load liposomes with doxorubicin but, i don't want to use the transmembrane pH gradient method, could there be other better method of doing the loading?

02 April 2019 4,967 2 View

Does anyone have a protocol for staining using Osmium tetroxide solution for electron microscopy?

I am planning to perform analysis of liposomes using TEM. Therefore, i thought of using Osmium tetroxide solution as a staining agent.

20 March 2019 6,137 4 View

Should heutagogy, as an instructionl approach, only be used in formal teaching & learning situations, or can it be used informally as well?

The main thing I am interested in here, is whether heutagogy is a valid instructional approach for non-formal teaching & learning situations. Please tell me what you think!

31 January 2019 5,972 6 View

Speed up the performance in DC-DC Power Converter?

Dear Ms, I have been thinking in how to Speed up the performance in DC-DC Power Converter?, because I have a systems which has 2 DC-DC converters, the first one is working in order to obtaing the...

30 January 2019 4,819 7 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View