Tony Heurtaux

6 Questions 33 Answers 0 Followers

Questions related from Tony Heurtaux

GDNA extraction from mouse tails for sequencing (MinION) ?

Hi everyone, it's now several weeks that I have problems for the sequencing of mouse samples with the MinION device (from Oxford Nanopore Technologies). For that, I extracted gDNA from mouse...

14 October 2021 8,326 0 View

Can somebody tell me why fatty acids are often complexed to BSA before cells? Why not directly treating with the fatty acid solution?

For example: "Fatty acid stock solutions (100 mM) were made by dissolving their sodium salts in 70% ethanol with gradual heating to 55◦C. 8% fatty acid-free low-endotoxin bovine serum albumin...

02 February 2021 6,675 3 View

Cholesterol stock solution for cell culture ?

Dear all, can somebody help me concerning the preparation of a cholesterol solution for cell culture ? Do I have to prepare a stock solution in ethanol and then dilute it in culture medium at a...

02 October 2017 8,068 4 View

Cre-Lox mouse model in vitro ?

Hello everybody, I want to use a Cre-Lox mouse model to study the behaviour of glial cells during a Parkinson-like state. In our in vitro model, we take glial cells from newborn mice to perform...

15 November 2016 5,026 3 View

Has anyone used Nrf2 transcription factor inhibitor(s)?

I am working on microglial cells (macrophages from the Central Nervous System). It's really difficult to do transfection (transient, siRNA) in these cells due to their phagocytic capacities. I...

21 May 2014 7,270 24 View

DNA extraction from mouse tails for future sequencing with the MinION device

Hi everyone, it's now several days that I have a problem for the sequencing of mouse samples with the MinION device (from Oxford Nanopore Technologies). For that, I extracted gDNA from mouse...

01 January 1970 8,457 0 View