I transformed a knockout yeast with a plasmid that contains a gen that complement the disrupted gene in the knockout yeast. They both are involved in glycosylation, so I want probe the functionality at the heterologous gen in the yeast.
You could probably perform a western blot for CPY.
Yes, I'll try CPY glycosylation. Thanks!
how big would be the shift if CPY glycosylation is removed?
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