Hi, You can share me your email or i will share you the link of the document where you can find the complete protocol including FISH

To detect and characterize the bacterial microbiome in tissue samples using FISH, start by fixing the tissue with a solution like paraformaldehyde to preserve its structure and nucleic acids, then prepare thin sections by embedding and slicing. Next, permeabilize the tissue sections using enzymes such as lysozyme or proteinase K to allow probe penetration through bacterial cell walls. Hybridize the samples, wash the samples to remove unbound probes....Finally, observe the samples under a fluorescence or confocal microscope.