Hi, I wanted to know how much percentage of proteases constitutes in an enzyme family
Read this article available on net.
A Brief Guide to Enzyme Nomenclature and Classification
Keith Tipton and Andrew McDonald
Article The Classification and Evolution of Enzyme Function
Enzyme classification is done by the Enzyme Commission, a body of IUBMB. Its web-page is https://www.enzyme-database.org/class.php, there you find a complete list of all enzymes that have an EC number assigned.
In general white opaque plates are recommended for luminescence, transparent for absorbance and black for fluorescence assays respectively. My question is if we can use black bottom plates for...
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I have to co-culture cancer cells with normal cells to check the efficacy to drugs target to cancer cells. In this regard, I am looking for a cell surface marker which can stain only cancer cells...
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I have to perform fluorescence based assays in 96-well plate using HEK-293 cells. The experimental procedure involves transfection in cells and then treatment with drugs for testing drug efficacy....
04 May 2019 5,056 10 View
I recently coated the 96 well plates with 0.1 % w/v (1:10) dilution of Poly-L-Lysine for seeding the HEK-293T cells. However, I found that the attachment of the cells to the surface is not...
04 May 2019 6,263 2 View
Enzymes are synthesised as zymogens and then make a transition to active form when required, is this true for every enzyme or are there any exceptions to that?
02 March 2019 1,554 4 View
I have expressed EGFP-N1 plasmid (1ug) in MCF-7 cells. Upon taking the confocal images under 40x oil I have seen the expression all over the cell? What is the possible reason for that? Can anyone...
01 February 2019 2,508 6 View
Why some cell lines are easy to transfect and some hard to transfect?
31 December 2018 9,583 3 View
Have to see protein-protein interaction in HEK-293 cells, where the donor GFP tagged protein and acceptor is RFP tagged protein. Please suggest me some good tips that I should keep in mind for...
31 December 2018 9,469 1 View
I have a vector pET23b with monomeric EGFP-6X His tagged cloned in that. The plasmid is transformed in BL21(DE3) cells. Kindly suggest me some good literature for the purification of the GFP-His...
31 December 2018 461 4 View
Transfection efficiency depends on a lot of factors among which the ''the cell line type'' is one of them? In general, which cell line shows maximum transfection efficiency and how much?
31 December 2018 3,502 6 View
I'm interested in learning about enzyme usage. Could someone explain the basics of working with enzymes? I understand they're different from standard chemicals, but I'm uncertain about the...
01 August 2024 5,460 5 View
I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...
30 July 2024 2,483 5 View
I am working on carbonic anhydrase immobilization into MOFs. I am facing problems with low enzyme loading.. The other issue is that when using p-NPA activity test to detect the activity of the...
20 July 2024 1,440 3 View
Bioinformatics tools like peptide cutter
15 July 2024 6,453 1 View
Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...
10 July 2024 3,050 5 View
كيف يساهم انزيم الكاتلاز في عملية التمثيل الضوئي في النباتات؟
09 July 2024 7,512 1 View
ماهو دور انزيم الكاتليز في النباتات؟
09 July 2024 5,824 2 View
what is the protocol for enzyme freeze drying?
09 July 2024 6,464 6 View
Hi We have alpha-amylase enzyme that written 5 units /mg protein on its container. How do I make 10 cc of enzyme solution with a concentration of 0.2 units/mL?
08 July 2024 2,285 2 View
How we should screen out the solid material left after 90 days of fermentation in making of garbage enzyme. Is there any standardized process.
17 June 2024 4,840 3 View