Due to problems with using ammonia as Ph buffer, Can somebody help me to select alternative Ph buffer to be used with cobalt chloride CoCl2 and sodium hypophosphite and sodium citrate in alkaline bath?
Did you tested ethylenediamine? However, you will probably need to change the stabilizer(sodium citrate) also.
Hello, I am trying to identify the formation of a synthesis product. Knowing the precursors, I focused on the identification of the H-C long-range coupling between sites (a/b) and (a/c) using...
19 January 2021 8,438 5 View
I would like to conduct a [1H-13C]- HSQC (optimized for one bond H-C connection) experiment. What would be the best default topspin pulse program and parameters set to use ? Thank you.
14 December 2020 6,956 5 View
Hello, I'am trying to run the 1H NMR spectrum of an ionic solution (Bromide Salt). the solution is too viscous so I had to randomly add a certain volume of deuterated chloroform, and then...
30 November 2020 8,569 1 View
I am running pilot test and the Cronbach's alpha is 0.71 for all of the items. However, when I come to analyze every section of the questionnaire individually, In found that it is too low. And I...
10 October 2020 6,213 1 View
I'm running DSC for a mixture of 2 polymers and I need help to interpret the heat flow curves
13 August 2020 2,993 4 View
Mohammad Salem from the Alexandria University claimed the authorship these three papers: 1. First Report of Fusarium verticilloides causing Banana Fruit Rot in Jordan 2. First report of cucurbit...
26 July 2020 9,647 3 View
After the field data has been collated using Likert-style instrument e.g 'Strongly agree,Agree,Neutral to Strongly disagree, , what is the appropriate test statistic to use ? (i.e easy to...
18 May 2020 5,039 4 View
If I have a set of input data and a set of output data as shown in attached file, Can a Neural Network predict an output if the input data out of the given input data? Example: if the new...
11 June 2019 2,899 9 View
Hello every one ,any recommended proofreading service website for thesis or paper editing ?
09 June 2019 7,162 13 View
Is there any tool used for A.I neural networking rather than WEKA tool ? Is there any good recommendations ?
25 March 2019 3,687 3 View
Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...
03 March 2021 1,499 2 View
Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...
03 March 2021 6,299 3 View
Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...
01 March 2021 2,215 2 View
Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.
01 March 2021 9,952 3 View
I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...
01 March 2021 9,015 2 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I have a tank which contains natural gas at 250 Bar and 293 Kelvin. I want to cool the gas in a dry-ice cooling bath to about 195 Kelvin. The gas will flow in a copper-tube through the bath with a...
27 February 2021 8,128 1 View
I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...
27 February 2021 8,483 3 View
I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...
26 February 2021 7,129 3 View
Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...
24 February 2021 5,749 3 View