You can probably count metastatic nodules on lung manually. If you have significant effect, you will probably see it by naked eyes.

Dear Sandeep , Thanks for your answer ..

Actually I can see the metastasis by naked eyes. But I has 5 sequence of slice per lung.Now how to quantify the metastasis by statistically 5 animal per group & 5 slides per lung.?

Either can take average of 5 slides consider one animal metastasis?

Is it correct way of statistics? or can do 5 animal x 5 slides= 25 slides SEM will be correct?

Please help me to understand in better way.. 

In that case u can take average of 5 slices per animal and it will represent metastasis in one animal

Rushi, Sandeep is right. Statistics should be done for no. of lesions/animal. Hope everything is going well. :)

If you have 5 consecutive sections of your lungs - make sure you do not count the same metastatic nodule twice.

Hope that helps.

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