Hi,

I am new to doing chromosome conformation capture and I have been looking at various protocols that are out there. Most of the protocols seem to make the libraries on the strep beads after the biotin pull down step by A tailing followed by adapter ligation and PCR amplification. I was wondering if it is also possible to generate libraries using the Tn5 tagmentation enzyme. If so, what would be an ideal Tn5 enzyme : DNA ratio?