Are single-cell RNA Seq libraries stranded?

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How can I add a book chapter and an article?

25 March 2024 5,284 2 View

What are students perspectives on school fights?

What do students think are the reasons for school fights

26 February 2024 4,633 5 View

How does DNA strand denaturation occur in LAMP?

I don't understand how BST manages to denature the DNA strand or how the primers bind to a double-stranded DNA in LAMP reactions. A constant temperature is applied, but for BST to displace the...

07 November 2023 8,041 2 View

What to do when my ANOVA analysis has a medium effect size but a non-significant p-value?

Hi everyone. I am running an ANOVA analysis, and there is an interaction with a medium partial eta squared value (.06). Still, the p-value is insignificant ( .11). I have calculated the sample...

06 June 2023 3,434 6 View

Starting problem with Xcalibur. Can anyone help me fix this?

Has anyone encountered this message when starting Xcalibur? Home Page: "The application has failed to create an instance of the compliancedatabase service. It will be shut down to comply with 21...

21 May 2023 4,858 0 View

How to do western blots to detect TCR signalling?

I would like to do western blots to look at early and later events in TCR signaling such as Zap70 phosphorylation, Lck phosphorylation, PLCg phosphorylation, Erk p etc.. I see in papers that cells...

07 March 2023 6,593 0 View

Why does guide-seq require custom Read 2 and index 1 primers?

I am trying to understand why the guide seq protocol requires custom read 2 and index 1 primers for sequencing on the MiSeq platform. From my understanding of the library prep method, if the P7...

30 March 2022 9,021 0 View

Do you know how to get access of "CLSI. 2018. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically,11th Edition?

I would like to have access to the pdf "Clinical and Laboratory Standards Institute. 2018. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically, 11th...

20 December 2020 2,467 4 View

Can someone help me optimize the pcr lamp test?

I am trying to optimize my PCR Lamp assay. work with BST 2.0. would it be interesting to join BST 2.0 with BST 3.0? My problem is that I can't identify with the Lamp Ct> 29. would anyone have...

08 November 2020 7,507 1 View

Is a Repeated Measures One-Way Anova apropriate to determine statistical difference in a time based experiment? Which post-hoc test should be used?

Hello! I developed an in-vitro gastrointestinal digestion of a fruit with 3 biological replicates of the colon area. That being said, I want to determine if there is statistical difference...

21 January 2020 4,298 3 View

Feedback defines the constitution of an organism?

“Here is a thought experiment. Let's place Rodolpho Llinas's jarred-brain on top of a body (Fig. 1). I bet Llinas would argue that his jarred-brain retains its own consciousness, and the android...

11 August 2024 2,483 1 View

Self-Organizing Superorganisms—as envisaged by Nenad Sestan (2018)?

The rate of glucose consumption by the neocortex is reduced by over 80% during anesthesia (Sibson et al. 1998), which disables the synapses (Richards 2002) that are inundated by glial tissue (Engl...

08 August 2024 3,118 0 View

Measuring the Intelligence of a Species?

Larger brains, which typically contain more neurons, store and transfer more information (Tehovnik and Chen 2015), but the precise relationship between number of neurons and information has yet to...

05 August 2024 1,238 2 View

How can i do multivariate Time Series forecast using MLP, ANFIS and LSTM?

I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.

05 August 2024 2,977 3 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

Need help with my research project on open source SIEM and machine learning?

Hello everyone, I am currently working on a research project that aims to integrate machine learning techniques into an open source SIEM tool to automate the creation of security use cases from...

04 August 2024 3,196 2 View

Swimming/space travel depends on the proprioceptive muscle spindles?

When the entire neocortex is ablated in rodents, although they are still able to swim, all the limbs move continuously and asynchronously (Vanderwolf 2006; Vanderwolf et al. 1978). Normal animals...

03 August 2024 835 3 View

What are the limitations and challenges of using machine learning for predicting concrete compressive strength in practical applications?

Machine learning (ML) has shown great potential in predicting the compressive strength of concrete, an important property for structural engineering. However, its practical application comes with...

03 August 2024 2,546 2 View

Some new emerging problems on application of RL for scheduling in IoT networks?

I have seen plenty of existing works on applied Reinforcement Learning (RL) policies for optimized scheduling in IoT networks including Q-learning, DQNs, and the newer ones including PPO for...

01 August 2024 8,754 2 View

How to Compress Information Neurally?

Samuel Morse, the inventor of the Morse Code, understood that certain letters in the English language occurred more frequently than others (Gallistel and King 2010). To deal with this, Morse used...

01 August 2024 4,456 2 View