Livia Jackman

4 Questions 1 Answers 0 Followers

Questions related from Livia Jackman

How to do western blots to detect TCR signalling?

I would like to do western blots to look at early and later events in TCR signaling such as Zap70 phosphorylation, Lck phosphorylation, PLCg phosphorylation, Erk p etc.. I see in papers that cells...

08 March 2023 6,594 0 View

Why does guide-seq require custom Read 2 and index 1 primers?

I am trying to understand why the guide seq protocol requires custom read 2 and index 1 primers for sequencing on the MiSeq platform. From my understanding of the library prep method, if the P7...

31 March 2022 9,022 0 View

Are single-cell RNA Seq libraries stranded?

I am trying to learn about scRNA seq library preparation methods and it is not clear to me if these libraries are stranded. Would really appreciate it if someone can explain this to me.

02 August 2021 7,708 2 View

Can sequencing libraries for HiC be prepared by tagmentation using Tn5?

Hi, I am new to doing chromosome conformation capture and I have been looking at various protocols that are out there. Most of the protocols seem to make the libraries on the strep beads after the...

21 January 2020 1,048 0 View