I am wondering if Salmonella enteritidis can transcribed a gene which is under lac promoter? Since most of the salmonella is lacking lac operon and I am not sure the specificity of the RNA polymerase to recognise different types of promoters.
The lac promoter has long been used in Salmonella species where it works as it does in E. coli. However since Salmonella has no lacI expression will be constitutive unless you also move in a plasmid (or F') that carries lacI.
The lac promoter has long been used in Salmonella species where it works as it does in E. coli. However since Salmonella has no lacI expression will be constitutive unless you also move in a plasmid (or F') that carries lacI.
John is correct, Salmonella species are closely related to E. coli and promoters will work well across species, but it won't be regulated in the absence of introducing lacI.
@John Cronan Thanks for your answer. Normally there only one RNA polymerase and its ability to recognize promoter is depend if there is a suitable sigma factor. Can this mean particular sigma factor in salmonella can recognize lac promoter. Is there any book or journal showing salmonella can express protein under lac promoter?
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