I would like to store adipose tissue (from Wistar rats) in RNA later but have heard that the reagent cannot be used to stabilise RNA in adipose tissue due to the high abundance of fat.
Dear Tarryn,
In fact i don't have experience using RNA later for adipose tissue, but i use it for storing rat's brain several time. As brain consist of considerable amount of fat i think RNA later generally works for adipose tissue.
Dear Taryn,
I work with human adipose tissue for my dissertation research, and I have found that RNA Later does work fine, but I find that just flash freezing a small piece of the tissue or directly homogenizing in Trizol works better in terms of RNA quality at the end.
Dear Taryn
I find that the snap freez is the best method for AT preservation.
Hi Tyrran
Actually there is no limitation in using RNA later for different tissues, scince rapidly permeates tissues to stabilize and protect cellular RNA.
For more detailes please see the attached file, containing the protocol of RNA extraction from adipose tissue and reagents.
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