Bronson A Haynes

2 Questions 4 Answers 0 Followers

Questions related from Bronson A Haynes

Are there any recommendations on how to culture human adipose microvascular endothelial cells so they do not loose their endothelial phenotype?

I have been trying to isolate human adipose microvascular endothelial cells from human omental and subcutaneous fat. I use CD34 and CD31 immunoseperation techniques for the cells to isolate out...

24 June 2017 1,418 5 View

Can someone help with a calculation of triglyceride amount in liver tissue?

I extracted lipids using Folch technique and evaporated the chloroform/methanol mixture and resuspended the lipid in 200-uL of DMSO. I then determined the concentration of triglyceride (mg/dL)...

29 April 2015 7,075 6 View