Tarryn Willmer

5 Questions 6 Answers 0 Followers

Questions related from Tarryn Willmer

Is it possible to transfect cells with a plasmid using DharmaFECT 1?

I need to transfect cells with a plasmid (GFP). I normally would use a transfection reagent such as lipofectamine or fugene but I only have DharmaFECT 1, which I use for siRNA. Do you think I...

06 February 2023 6,384 4 View

Assessment of gut microbiota (16S rDNA) using pyrosequencing?

Has anyone ever used 16S rDNA pyrosequencing to identify gut microbiota populations? We have a PyroMark Q96 and I would like to adapt the sequencing protocol to assess gut microbiota in faecal...

19 August 2021 4,784 3 View

What is the best online tool to find transcription factor binding elements in a DNA sequence?

I am trying to locate GREs (glucocorticoid response elements) within a gene sequence. I would like to find a user-friendly online tool to help me find full GRE consensus as well as half sites, if...

09 January 2019 5,681 2 View

DNA quality and purity for pyrosequencing experiments?

Is there a 260/280 ratio cutoff for DNA that is to be used for bisulfite conversion and pyrosequencing? How pure does the DNA have to be?

31 July 2018 9,649 1 View

Can RNA later be used to preserve adipose tissue?

I would like to store adipose tissue (from Wistar rats) in RNA later but have heard that the reagent cannot be used to stabilise RNA in adipose tissue due to the high abundance of fat.

22 June 2017 4,023 5 View