Can Ribitol be substituted by any other standard for LC MS analysis?

16 April 2022 1 8K Report

Hello,

I'm attempting an LC-MS analysis for a plant methanolic extract for the sake of polyphenol and terpenes quantification. I'm following a protocol used by Nivea M. et al., (2019) in which they used Ribitol as an internal standard.

I was wondering if I can substitute Ribitol with another standard and if I can then what are the possible choices I can use?

Thank you

Yasuhiro Nishida

Hi.

I see that many papers are analyzed using external standard methods.

Are you concerned about the recovery rate from the analyte?

I think you have a few compounds to choose from following articles.

baicalin

Article HPLC Determination of Eight Polyphenols in the Leaves of Cra...

catechin and kaempferol.

Article Quantification of major phenolic and flavonoid markers in fo...

quercetin

Article Determination of phenolic acids and flavonoids in Rhinacanth...

biochanin A

Article Chromatographic Determination of Phenolic Acids and Flavonoi...

Anyway, of the polyphenols that would not be in your analyte, you can use those that are relatively close in nature and available in high purity. I believe you can solve this problem by selecting the appropriate one that is not contained in the analyte.

And I believe the topic someone else posted earlier may be helpful...

https://www.researchgate.net/post/Could_anyone_suggest_me_about_suitable_internal_standard_for_citrus_polyphenol

Good luck！

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Science method

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