HPLC system been sitting for a long time no use, I performed a whole system flushing in accordance with manufacturer manual and refilled seal wash solution. However, I noticed that the level of seal wash solution rises everytime the system is working. what could be the problem?
the system is a younglin YL9100 HPLC system.
You have a seal wash leak and your mobile phase is leaking through the worn seal(s). Please have your HPLC pump professionally serviced. Regular maintenance and cleaning are required for operation.
- The instrument can not be used for sample analysis when a seal-wash leak is present.
Note: For any future posts on "HPLC" please provide the exact make. model and type of HPLC module, the mobile phase used in it, the flow rate and other basic information to help users put your observations into context. Help is most likely to occur when you share basic information in your initial posts (or requests). Please contact some at your school who has professional experience in HPLC to assist you with these types of questions (and/or hire the local instrument service tech to perform the needed services on your instrument).
Agree with William. You have a leak from high-pressure zone. At least, seal needs replacement by service engineer.
Your results will not be correct in these conditions because it reduces flow through the column. You may see chromatogram, but retention times will be greater, selectivity of separation may differ as well.
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