Can crude enzyme be used for immobiliazation?

Can crude enzyme be utilized as immobilized enzyme for industrial application if the crude enzyme shows high activity?

13 July 2020 8,672 4 View

From where can I get microglia cell line like N9 or BV-2 in India ( preferably in Kolkata)?

From where can I get microglia cell line like N9 or BV-2 in India ( preferably in Kolkata)?

05 November 2019 7,719 2 View

How to conjugate a dye to the N-terminus of poly-glutamic acid peptide?

Hi! I am trying to conjugate a dye in its NHS ester form to the N-terminus of poly-glutamic acid peptide ( which is already available in the form of a white solid). I tried 3 times and am tring...

27 June 2019 8,798 3 View

Is it necessary to purify PCR products before cycle sequencing?

I will purify the cycle sequencing products using EDTA/EtOH precipitation method before loading to 96 well plate.

30 November 2018 6,932 4 View

In case of Box behnken design reduced quartic model, do I need to mention all the insignificant terms in the ANNOVA table and polynomial equation?

When I try to reduce those terms the R2 value reduces. As a result I have many terms whose sum of squares and df values are 0. The p value and all other values fall in range.

22 May 2018 8,088 4 View

How to measure voltage dependent strain of a thin film without using electric field dependent XRD???

because we have not any facility of electric field dependent XRD but all the papers suggested this electric field dependent XRD. If anyone can explore any idea then please help me

15 April 2018 263 2 View

In an XRD data if the peak are same with ICSD data but intensity is not matched with ICSD data then is anything worng???

My XRD data for NdNiO3 is similar with ICSD data but intensity are not matching with ICSD data

11 March 2018 8,823 4 View

How a core flood experiment for conformance control can be designed?

The limitation we are currently having is that cores of length upto 6 inch can only be used in our system and we can not prepare composite cores.

03 September 2017 9,093 2 View

Can zero field cooled MH loop measured after some times (few months) for same magnetic sample at same temperature be different?

17 February 2017 6,694 5 View

PLD Vs RF sputtering?

How PLD and RF sputtering are different over each other ? Or why one should choose PLD over sputtering except depositing complicated oxides (e.g: super conductors or perovskites). It is well known...

12 October 2016 1,464 4 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

What are anisotropic electrical and optical properties of a semiconductor due to?

In some materials, properties such as band gap, thermal conductivity differ along different crystallographic axes. What are the basics?

01 March 2021 1,923 3 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

No title

25 February 2021 5,713 1 View

Where can find Medical Image Data Sets for Machine learning research project?

I want to work on medical image(disease) detection and classification. Where can I found Image data sets?

24 February 2021 3,130 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

What is your preference for journals for publishing in the field of Hyperspectral Imaging or Remote sensing?

I believe that we need to share our experience in publishing in the field of Hyperspectral Imaging or remote sensing? What is the best journal that you publish with? What is the worst experience...

23 February 2021 3,411 4 View

How can we determine the band gap of a composite material that is made up of a composition of direct and indirect band gap semiconductor materials?

I have a direct band gap material and an indirect band gap material. If I made a composite of both through physical mixing. Then how can I determine the band gap of such a material as the Tauc...

17 February 2021 8,104 4 View

CT image preprocessing in python?

I have a 3D CT image of a car engine in raw form with no header information. When loaded into numpy array as 16 bit unsigned integer, I noticed that the values range between 0 to 52000. Is this...

15 February 2021 4,736 3 View

Despite single-cell RNA, how can I characterize a new cell subtype/phenotype?

A new cell subtype was found out only during regeneration phases after a cuprizone-induced demyelination model. How can I characterize this cell? It is important to highlight that was already...

13 February 2021 1,245 1 View