Can puromycin interfere with calcium release?

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RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

What are the differences and similarities between academic research and evaluation?

As a graduate student at the Arizona State University in the Mary Lou Fulton College of Education, I am beginning my Capstone process. In one of our classes, we have been asked to develop a...

15 May 2024 3,514 1 View

How can I improve Gibson Assembly's efficiency?

In my undergraduate research project, I require an integrative plasmid containing GFP with an antibiotic resistance that can't be kanamycin. In our lab, all of our integrative-GFP-containing...

14 May 2024 9,861 3 View

How to improve 260/230 ratio in rna extraction for PCR?

The 260/280 ratio is ok between 1.8 and 2.0 however we were unable to advance due to the 260/230 ratio

13 May 2024 7,643 3 View

MorphoJ is not giving me the results of Canonical Variate Analysis. What I do?

When I request MorphoJ to do a CVA, it gives to me only the graphics, but no results.

27 March 2024 1,672 1 View

Why the best blocking solution to blot?

We already used milk: 5% to 1 hour, 7% to 2 hour, 10% to 1h, 5% overnight, but, , in all blockages it still marks the entire membrane, every run, there is a large band with no specific mark HELP

20 February 2024 7,346 0 View

Can scholarly journals be used in triangulation?

I am using triangulation for my dissertation and need to know if scholarly journals can be used

15 February 2024 7,084 0 View

Is my research useful?

12 February 2024 7,142 3 View

What are the two main categories of diagnostic tests for HDV and what distinguishes them?

In relation to the systematic review and meta-analysis of Zhenzhen Pan et al. (2023) about the Diagnostic Efficacy of Serological Antibody Detection Tests for Hepatitis Delta Virus.

06 February 2024 359 4 View

What is the preparation of culture medium for C6/36 cells?

Hi, i´m working with C6/36 cells Do you have a manual for preparing growth and maintenance medium for C6/36 cells? What is the CO2 concentration and temperature that you use in the incubation? please

04 February 2024 7,525 1 View

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under op microscop?

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?

07 August 2024 2,132 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds?

XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds

04 August 2024 3,019 3 View

Looking for help on sem image analysis?

Hello I am conducting a microstructural analysis of a soil treated with lime. The following sem images are of the untreated s1 and treated soil s3. The untreated soil contains quartz calcite...

01 August 2024 572 0 View

How to add Cr parameter in autodock?

When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!

29 July 2024 488 0 View

(treating astrocytes with LPS) Has anyone observed the recovery of astrocytes from LPS injury, a few hours after the treatment?

or can you recommend articles showing the differential effect of LPS in a time dependant manner?

28 July 2024 2,547 1 View

Can we eliminate the stress singularity at the tip of the crack by manipulating the elastic constants?

The aim of the research here is to prevent the propagation of the crack in the fabricated elastic medium with useful applications.

25 July 2024 9,976 3 View

A twin-shaft kiln or a rotary kiln for lime production 1 class? if compressive strength of limestone is low 60 MPa?

My research work is related to the calcination of limestone. I have a complex question and would like to consult with experts in this field. Could you please advise which kiln to choose (a...

18 July 2024 1,390 2 View

What is the difference between alluvial and fluvial?

I have made an unpleasant discovery that I seemed to use these terms incorrectly. 'Alluvial' seems to mean "out of the river channel", flood-plain' etc. While all these cross-bedded...

15 July 2024 2,073 3 View

How to calculate percentage cumulative drug release?

How to calculate percentage cumulative drug release when, Total volume of dissolution media = 250ml Added formulation was equivalent to 10mg of drug Sample withdrawn = 1ml each time Sink condition...

15 July 2024 4,192 1 View