Beatriz Duran

4 Questions 8 Answers 0 Followers

Questions related from Beatriz Duran

Problems with the attachment of SH-SY5Y cells during the MTT assay?

Hello, I am conducting MTT assays on various cancer cell lines. While I have no problems with any of the other lines, I have seen that a good number of the SH-SY5Y cells I plate for the assay...

30 October 2022 1,594 6 View

Is it convenient to normalize the results from a free-radical formation test to the number of viable cells in the culture (e.g. MTT data)?

I'm conducting tests to measure free radical formation (using H2DCFDA), following exposure of my cultures to various toxic compounds. Should I normalise the readings I obtain to those I got from...

19 March 2015 7,873 7 View

Explants on collagen drops- Is there any protocol to ensure attachment of the collagen drop on the coverslip?

I am trying to set up some explants on collagen drops on glass coverslips, but these drops come off over time. Is there any protocol to ensure attachment of the collagen drop on the coverslip?...

15 April 2014 4,320 3 View

Can puromycin interfere with calcium release?

Puromycin and calcium release

30 October 2013 2,523 3 View