Capture of oleophilic solid polymers present in water solution ?

I am looking for articles, coferences, publications or thesis concerning the capture of solid oleophilic polymers in solution in water. Thanks Thierry Le Blan

01 January 2021 5,798 3 View

Phylogenetic study on African Pieridae and Papilionidae ?

Does exist a modern phylogentic / molecular study, or being in preparation, on the Pieridae and Papilionidae including African ones (with a large spectrum including at least all the "species...

11 August 2019 9,571 6 View

We are looking for a catalyst to oxidize NO to NO2 ?

We exclude to use an ozonizer as the sample gas composition includes SO2 in significant portion (several hundred of mg/m3). During tests with an ozonizer, we observed on a short term running time,...

21 November 2017 1,796 4 View

Does epinephrine increase the toxicity of dental local anesthetics on the CNS?

10 July 2016 7,981 8 View

Is there an interest in the creation of a working group on Cenozoic supergene mineral deposits across Africa?

The Royal Museum for Central Africa (RMCA, Tervuren, Belgium) has an acknowledged expertise on mineral deposits in Central Africa: Cu-Co and Mn in the Katanga, Sn-Ta-Nb in Eastern DRC, Rwanda and...

15 September 2015 7,997 11 View

What methodology people are using for cytokines detection in cats?

Hi Everyone, I am looking in detecting some cytokines (i.e. IL1 or 6...) in feline blood and I am looking for some advice. So far I have been looking at ELISA as a possible method, but could not...

24 February 2015 3,037 4 View

What is your opinion on quantifying double labeled immunofluorescence markers or adjacent slides IHC?

Like to know other researchers opinion on quantifying some immunohistochemical/immunofluorescence data, I double labeled some markers using IF on FFPE tissue samples, I also have the adjacent...

11 June 2014 9,438 4 View

How can I efficiently quench external fluorescence?

I am trying to set up a phagocytosis assay using CFSE-labelled apoptotic thymocytes as particles. I want to quench external fluorescence to make sure my FACS measurements really reflect...

13 April 2014 5,905 3 View

I am looking for a human B cell line that proliferate slowly and could be induced to proliferate further by BCR signaling

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13 December 2013 8,366 11 View

How to perform an in vitro phosphorylation of recombinant proteins?

We study the properties of a complex between two proteins. We have recombinant forms of these proteins purified from E. coli. We suspect that the phosphorylation of one of these proteins...

25 September 2013 4,981 18 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View