Yes they can, but this depends on the sample. Not all samples will grow on 2D. For example we have been given 4 renal cell carcinoma tumour samples from a surgeon and only 2 of them were able to grow on 2D.

epithelial cancer cells do grow on 2D, but we often have overgrowth of the fibroblasts in the sample

Yes, they can, but this depends on the sample.

Not all samples will grow on 2D.

but mostly lungs cells grow on 2D