Viramita Windy

3 Questions 3 Answers 0 Followers

Questions related from Viramita Windy

Can primary dilution be done using milliQ water?

I want to dilute IDT primers for gradient PCR but I don't have Nuclease free water yet. or are there any other suggestions? thank you

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Which part of adipose tissue should be taken from the sample that has been crushed with liquid nitrogen?

I am working with wistar rat adipose tissue, I want to extract RNA from the adipose tissue for gene expression in the process of fat metabolism (oxidation). when I crush it with a mortar using...

30 April 2025 1,340 2 View

How to sterilize surgical instruments for RNA isolation from mouse adipose tissue?

I need to isolate RNA from mouse adipose tissue using the GeneAid kit. There are some guidelines that mention using DEPC to remove RNAse. But DEPC stock is very difficult to find, are there any...

20 February 2025 1,764 3 View