We are facing a problem in detecting polysorbate 80 in aqueous suspension as it is not detecting in HPLC. If we are spiking PS 80 in the vehicle, it is observed that the area is very less compared to the standard.
We are facing issue in vitamin B12 assay as we cannot correlate by mass balance in force degradation. We are having HPLC with UV Detector method.
08 September 2018 1,718 7 View
I am looking for a method of estimation of propylene glycol alginate in pharmaceutical solid oral formulation.
11 December 2017 3,990 2 View
We have followed a literature method for NaHSO3 and NaHSO4 Detection by HPLC (Literature attached) in suspension formulation. Though we are receiving the peak of NaHSO3, unfortunately no peak of...
10 November 2017 2,127 3 View
Can sodium sulfate or sodium bisulfite affect the detection of polysorbate 80 in formulation? We have a formulation which cannot be detected in presence of NaSO4. What can be the reason? How can...
10 November 2017 9,111 10 View
Can anyone please share a method for mineral oil estimation in ointment. We already tried GC-FID.
05 June 2017 7,571 8 View
We are estimating polysorbate 80 by HPLC, initially we were getting expected result, but the sample sample when we analysed after 2 months, we are getting higher values. Can anyone say why this is...
04 May 2017 4,757 7 View
We need to determine cross linking in polymer. Please let me know the method for that.
04 May 2017 4,941 8 View
I need to determine the purity of XPRD pattern.
08 September 2014 9,142 5 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Are there any suggestions or insights you can provide to help address this problem
06 August 2024 1,443 2 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
Mobile apps can be a powerful tool for enhancing academic performance, how can we use mobile apps for improving academic performance
04 August 2024 9,492 0 View
Regarding a model for simulating battery charge and discharge, what do you consider to be high fidelity? What is the acceptable percentage of error (regardless of the metric)? Could you suggest...
03 August 2024 5,358 0 View