How to determine Vitamin B12?

We are facing issue in vitamin B12 assay as we cannot correlate by mass balance in force degradation. We are having HPLC with UV Detector method.

08 September 2018 1,637 7 View

Do anyone have method for propylene glycol alginate estimation?

I am looking for a method of estimation of propylene glycol alginate in pharmaceutical solid oral formulation.

11 December 2017 3,893 2 View

Detection of NaHSO3 and NaHSO4 by HPLC. Where is the problem?

We have followed a literature method for NaHSO3 and NaHSO4 Detection by HPLC (Literature attached) in suspension formulation. Though we are receiving the peak of NaHSO3, unfortunately no peak of...

10 November 2017 2,039 3 View

What is the compatibility between polysorbate 80 and sodium sulfate?

Can sodium sulfate or sodium bisulfite affect the detection of polysorbate 80 in formulation? We have a formulation which cannot be detected in presence of NaSO4. What can be the reason? How can...

10 November 2017 9,031 10 View

Mineral oil estimation in ointment

Can anyone please share a method for mineral oil estimation in ointment. We already tried GC-FID.

05 June 2017 7,496 8 View

Polysorbate 80 quantification

We are estimating polysorbate 80 by HPLC, initially we were getting expected result, but the sample sample when we analysed after 2 months, we are getting higher values. Can anyone say why this is...

04 May 2017 4,683 7 View

How to determine cross linking in polymers?

We need to determine cross linking in polymer. Please let me know the method for that.

04 May 2017 4,819 8 View

Can anyone discuss how to determine the purity of XPRD pattern?

I need to determine the purity of XPRD pattern.

08 September 2014 9,067 5 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Microstrip antenna design tutorial of HFSS 3D layout module ?

I have designed a 24 GHz rectangular antenna on HFSS 3D model and got desired results while desing the same antenna with same dimensions I am getting resonance frequency at 27 GHz I am...

28 February 2021 4,426 2 View