Can Phusion polymerse use RNA as a primer for DNA synthesis?

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In a 'drive fed by DC-DC converter' system simulation we need to calculate load (DC-link) current. Is it possible without the info of DC-link voltage?

Controlled drive can be DC or AC. Drive's converter is ideal. DC-DC converter is fed by ideal voltage source. DC-DC converter provides DC-link voltage stabilization on a constant level. The...

26 January 2024 4,458 2 View

What type of filter should be used when printing with titanium powder in an SLM printer?

Dear Colleagues! I would like to know your advice about printing with titanium powder in an SLM printer. Will a HEPA filter be enough to filter the gas from residual printing products when working...

20 August 2023 9,837 1 View

Recent recommendations for using posterior latent-class membership (LCA) probabilities in regression as DV?

Dear all, I am trying to find the least biased approach to connect results from latent-class analysis with covariates. However, I do not focus on class assignments... Instead, I would like to use...

03 August 2021 2,944 2 View

How to measure experimentally conductivity electron mean free path?

Dear colleagues, can anybody suggest how to measure experimentally CONDUCTIVITY ELECTRON MEAN FREEE PATH (λ) in the thin metallic film? First of all, I'd like to know how it's change with the film...

01 January 1970 3,513 1 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View