Yes you can but you must be aware of the power of pcr to amplify. Every 10 cycles of pcr is 1000 times more product so if re amplifying you should dilute 1:100 and amplify 1ul of the diluted product and run a range of cycle numbers. Set up 6 tubes of pcr and remove one tube after 12 cycles,one after 14 cycles etc to see where the best amplification happens. If you run too many cycles you will get a high molecular weight smear instead of a clean band . This smear is product annealing with other product molecules and making longer products
Yes, but dilute your pcr amplicon first to make the next PCR anplification cleaner and more efficient.
Of course it could be used but it is nessecarry to dilute it as the ratio of 1:100 or 1:1000 to get clear band on next PCR.
Good luck
Yes you can after sample dilution but is preferable that you are using for both PCR an high fidelity polymerase because otherwise you have some risk to introduce mutations.
good luck
Manuele
Yes,but you should dilute 1:100 and amplify 1ul of the diluted product and run a range of cycle numbers.
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