Surajit Kar

12 Questions 17 Answers 0 Followers

Questions related from Surajit Kar

Showing non specific band using same primer for different tissue of single individual (Chicken).

I have got the non specific DNA band with the same primer in 1% agarose gel when DNA is isolated from tissue of chicken rather than blood (But when i use only blood of chicken i have found single...

01 January 1970 1,208 3 View

Need Universal Avian (Bird) primer.

Any one can give me the sequence of Universal bird primer with proper reference. Any help would be appreciated Thanks in advance

01 January 1970 1,912 2 View

How to standardize annealing temperature for this primer?

I am trying to standardize the annealing temperature of a Primer. In 53 degree,a clear band on has been found at proper position (500 bp to 600 bp) but a non specific band appeared within 300 to...

01 January 1970 5,310 3 View

How to pack PCR products for sending for the purpose of sequencing???

PCR products evaporate when sending for sequencing (it was send in a ice pack and every tube was sealed well by paraffin). Please suggest how to pack sample (PCR products) for sending ?

01 January 1970 3,379 3 View

Can PCR product used as a templete?

Is it possible to use pcr product as a templete without purification?

01 January 1970 989 8 View

Wash in 100% ethanol rather 70% in DNA isolation effect in sequencing results??

In DNA isolation, by mistake i used 100% ethanol wash in place of 70%. DNA band in agarose gel appeared fine enough. Is there any chance of getting low quality sequencing result of those samples?

01 January 1970 3,966 9 View

What should be the Query cover % in BLAST match?

What is the minimum accepted query cover percentage(%) range in BLAST (NCBI) with greater than 90% identity match? Thanks in advance.

01 January 1970 1,517 1 View

Is DNA concentration and 260/280 ratio good indicator for further processing eg. PCR and sequencing??

Is DNA concentration and 260/280 ratio good indicator for further processing like whether proceed for PCR and sequencing?? In some cases i have found that even when DNA concentration and 260/280...

01 January 1970 7,060 1 View

How much % identity is reqired to define same species?

How much percent identity is required to define same species (with E value =0 and query cover grater 95%) in NCBI blast match ? Thanks in advance

01 January 1970 9,910 4 View

How to perform time series analysis for "air temperature"?

I have time series data regarding "air temperature" collected from experimental field and in parallel i have also collected the air temperature from local meteorological department for 12 months....

01 January 1970 1,129 3 View

Significance test required after Jacob selectivity index??

I am using Jacob's selection model to determine foraging preference of insects and i have already got the result and value ranged between -1-+1. But i am confused about that is it necessary to...

01 January 1970 8,310 5 View

How to read chromatogram(.ab1) file containing mixed template?

Sample contain mixed template.In that case how to read chromatogram file (Sanger sequencing)?

01 January 1970 9,985 6 View