I would identify all strains of E. coli in groundwater and surface water samples. I am not sure if PCR can help me to do this, and what do I need?
Thanks
This may help.. http://www.atlantis.ulaval.ca/publications/fichiers/Nine%20PCR%20tests%20for%20E%20coli.pdf
If not use general 16S primers to detect all bacteria then sequence.. Or if you have the resources go for NGS..
To begin with, your PCR should be able to detect E. coli in general and not any serotypes in particular.
Yes PCR is the one of the simplest method to detect all the strains however the template DNA should the pure and need to optimize the PCR parameters as well as the PCR conditions. PCR can identify the even if you have a single bacterium but isolation of DNA and purification is most important
So thankful for your response.
As I understand, each strain of E. coli has different primer. However, if I want to know all strains of E. coli in one sample, how many primers do I need?
Or do I have to use another technique rather than PCR?
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