One of the research papers have used HEK cells instead of neuronal cell lines like N2a to study the effect of SOD aggregates causing toxicity.
Is it possible to study non-tissue specific cell lines to study the tissue related mechanisms?
Hey All! I am wondering what might be wrong with my band structure. I did the calculations using VASP and plotted the results using Origin. Although I have tried changing various input...
25 July 2024 2,920 11 View
Hello all, i am working on Chilly anthracnose disease and the pathogen involved is colletotrichum capsici but the problem is occuring while subculturing, i can isolate pathogen from chilly fruit...
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Hello everyone, I am conducting a meta-analysis to find if x has superior effect than y on the outcome. I used the SMD method and I got a significant moderate heterogeneity of a value I2:...
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Hello everyone, I am conducting a systematic review to see if treatment x is superior in its effect than treatment y. Since most the studies I found did not report interaction effect, I was...
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I require recent UWB antennas manufactured
15 May 2024 3,888 1 View
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I try to explaim better. I have the scores for 10 items. Each item score are decided between the accordance of two persons (say, e.g., a therapist and a patient). Therefore, there is much more...
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usgs has not given any scaling factor values to be applied for level 1 products. but it also doesn't make sense for satellite zenith angle to have values from 1-2768.
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Hello everyone, I am writing a systematic review about if X experimental group has superior effect than Y comparison group on outcome. Please note that the lower the scores of the outcome, the...
19 April 2024 1,812 1 View
I have three field margin types (grassy/shrubby/tree) and each type have 5 sites. I need to calculate Shannon index for insect counts from each field margin type.... Q1. In my case how should I...
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I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
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Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I received an e-mail invitation to join the editorial board of Clinical Cardiology Updates. While I have published a few articles related to cardiovascular disease, there are lots of colleagues...
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After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
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I have a theoretical framework for preparing a interview guide to assess the unmet care needs of a sub population of chronically ill patients and their caregivers(dyadic interviews).
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I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
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I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
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