Megha Shri N

5 Questions 11 Answers 0 Followers

Questions related from Megha Shri N

Why am I not able to get a clone of gene tagged with GFP?

As previously discussed, I redesigned the primers. I tried triple ligation, it did not work. So, the gene of interest was restriction digested from the vector containing gene and was amplified...

14 January 2014 3,502 8 View

What is the neural mechanisms involved in perception in both normal people and hallucinating people?

EBA - Extrastraiate body area is one such area involved in perception in brain. Is visual perception a single modality or a multiple modality involving other sensory modalities? If we want to...

08 August 2013 9,346 2 View

Can one use an alternate cell line like HEK (Human Embryonic kidney) to study the inclusion bodies caused in brain?

One of the research papers have used HEK cells instead of neuronal cell lines like N2a to study the effect of SOD aggregates causing toxicity. Is it possible to study non-tissue specific cell...

08 August 2013 222 0 View

If I want to induce oxidative stress using H2O2, at what conditions should I induce it on neuroblastoma cell lines?

If I want to induce oxidative stress by adding H2O2, what percentage of it should be added and at what temp? if I am doing imaging of cells before adding the H2O2, continue adding H2O2 directly...

05 July 2013 6,963 12 View

SNP in GABRA2 gene, I retrieved a sequence from NCBI. When I digest the sequence from Hinf1 enzyme in NEBCutter, it's not getting digested. Why might this be?

RFLP technique for GABRA2 gene in Alcoholism. Its rs279845 SNP in GABRA2 gene. This is the sequence which I retrieved from NCBI-SNP database....

21 June 2013 9,003 3 View