Hiii!

My answer is yes

Please note on the following points

1. Make sure that there is sufficient amount of cells to do Elisa, because there should be a detectable amount of intercellular protein to be bound detector antibody

2. Use any cell lysis buffers to lyse the adherent cells and centrifuge, now the supernatant will have the intercellular protein of interest. Take the supernatant and apply it on freshly primary antibody coated 96 well plate so that your protein of interest will be bound by primary coated antibody and other contents will be washed away during wash step

Hope this will be useful

Hello Hadeel Khalouf

You can try using In-cell ELISA protocol. Please refer to the link below for a detailed protocol from Abcam.

https://docs.abcam.com/pdf/kits/in-cell%20elisa.pdf

Hope this helps !

Best.

thank you Sandhya Vivekanandan and Malcolm Nobre ! that was really helpful ! =)