I need to measure the protein concentration at 280 and 370 nm but since the volume is small I can't use on the normal UV quartz cuvettes. Is there any good alternative?
Search for the make of your elisa plate..you will find all the information
@JJ.. thanks but i m using corning plates.. and they are polystyrene plates which gives auto absorbance ..since i have tried using that... and the values are 3.0 so ...
nanodrop UV spectrometer (2 uL read volume). They are pretty common.
http://www.nanodrop.com/
There are actually a lot of solutions out there. Try searching for dip-in UV/Vis heads with attached fiber optics (for example: DIPUV-Mini, http://www.wpiinc.com/blog/2013/05/01/application-notes/dna-rna-quantification-using-dipuv-mini-and-a-tidas-spectrometer/). For quantification you will have to do some math afterwards: http://www.labautopedia.org/mw/index.php?title=UV/VIS_Spectroscopy_in_Microplates ... good luck!
Normal plates cannot be used at 280 and 370 nm as they have more than 2.0 to 3.0 OD. You have to use UV compatible ELISA plates. If sample is small and you have or nearby Institution has nano-drop that you can use.
may be use this equation when measure OD AT 260nm and 280nm
protein at mg/ml=1.55*OD280-0.77*OD260 AND must be ratio between reading OD280 and OD260 not more 0.6
Nano-drop that you can use.
please find the attached files
http://www.nanodrop.com/
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