I'm wondering if naive SH-SY5Y cells can secret Aβ, and can I detect it by western blot or ELISA?
Or I can only find Aβ from APP transgeniced SY5Y?
It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...
17 July 2024 3,371 0 View
Is there any protocos to produce the medium for single hybridoma cell culture without the feeder cells layer in the plate(Co-culture)?
20 May 2024 8,214 2 View
The development of mRNA vaccines on mice and rabbits?
18 May 2024 7,475 2 View
We have constructed lentivirus transduced U251 cell lines to express (1) EGFRvIII (its amino acid sequence is MRPSGTAGAAFLALLAALCPASRALEEKKGNYVVTDHG..., which is identical to the sequence...
14 April 2024 2,370 1 View
NO
11 March 2024 3,725 1 View
It is a common belief that extreme weather and climate events increased in frequency and intensity over the last century or half a century, a time period that saw a rapid global climate warming....
16 February 2024 3,727 10 View
Does anyone know where I can find the data of measured above-ground biomass? I need the data of forest biomass measured on the spot for training and learning. If possible, I can also invest money...
12 November 2023 7,845 3 View
How can we achieve the effect of the added magnetic field on the electrolysis process during molten salt electrolysis?
01 September 2023 4,416 2 View
I'd like to know what's current progress of 'synthetic circadian clock' or 'synthetic oscillators'. Indeed, I would like to investigate methods to regulate protein expression (e.g., transcritpion)...
24 August 2023 8,165 1 View
Weather stations are key facilities to record long-term change in surface air temperature (SAT) in land. However, some weather stations are located in or near cities, and they may suffer from...
20 June 2023 8,860 2 View
I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?
29 July 2024 3,138 2 View
I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...
17 July 2024 3,785 9 View
hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...
16 July 2024 9,044 10 View
Hey! I aim to generate a transgenic knockin zebrafish line that mimetizes a genetic condtition that leads to a certain disease on human. To do so, I need to insert a codon for mutagenic aminoacid...
14 July 2024 6,240 0 View
I placed mice in metabolic cages to collect urine samples for analysis. However, the majority of the samples have dried up. Is there any way to retrieve the urine in those tubes without...
14 July 2024 6,724 0 View
I am preparing cancer cell conditioned media to study the effects of substances secreted by cancer cells on other cells. In some papers, conditioned media is prepared using FBS-containing media...
11 July 2024 7,063 1 View
Hello, So I have mouse brain tissue MS data from one of my predecessors and I have human serum data from the hospital that I collaborate with. We are trying to find markers for the diagnosis of...
11 July 2024 8,484 0 View
Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...
10 July 2024 3,050 5 View
What is the secret behind the success of the researcher named Dariusz Prokopowicz who has a "Research Interest Score" of 11,228 and has 2,620,101 reads in ResearchGate. How was it possible for him...
06 July 2024 2,963 0 View
Has anyone tried co-culturing and SH-SY5Y cells with human astrocytes and differentiating ? I am planning to seed astrocytes, let them grow for 3 days and introduce SH-SY5Y cells into it. Ideally,...
01 July 2024 243 2 View