I was wondering if somebody could tell me if indeed bacteria in particular those involved in xylitol production that secrete enzymes extracellular, also secrete NADPH extracellular to power the enzymes.
- for example - xylose reducatase from fungi/yeast needs NADPH to function.
If so how do they do this? - what transporter ? - any papers that talk about this i couldn't find any?
thank you
That would depend on whether the bacteria is Gram(+) or Gram (-), as well as on whether they possess the Sec or the Tat (or both) secretory systems. You can refer to "Bacterial Secretion Systems" by Green, E.R. & Mecsas, J (2016).
It would be hard to imagine that bacteria has evolved a mechanism to secrete NADPH out of the cell, because the cell needs it for biosynthetic reactions (anabolism). In fact, the cell has evolved mechanisms to keep the [NADPH]/[NADP+] ratio high intracellularly. If the cell secretes anything related at all, it would be NADH, not NADPH. The cell needs NAD+ for catabolism (breakdown reactions) to generate ATP. In fact the cell has evolved ,mechanisms to keep the ratio [NADH]/[NAD+] low inside the cell so it can have the oxidative power of NAD+ ready whenever needed for catabolism.
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