And afther to read the expression this same labeled RNA can be quiantified by densitometry in a gel?
Dear colleagues, In our XPS instrument, most of the time when we acquire the Pd3d region (for a wide range of materials containing Pd) it overlaps with a strong signal from Ca2p. Nonetheless,...
10 November 2020 4,487 4 View
Hi all, I am working with weka (software for machine learning). I have two sets of data, TRAIN and TEST. 1. The easiest question (I think). Is it possible to train modeland apply it to the TEST...
05 November 2020 3,317 3 View
Hi everyone, Im studying the relation between social media habits of customers and their risk aversion in order to create a model of machine learning to know which investment product fits better...
12 October 2020 5,975 3 View
The question I'm asking is to clarify if there is a link between Emotional Intelligence and the HR Industry if there is a link, can we increase the work performance of people in the said industry...
17 August 2020 1,797 12 View
I need to purchase both moth species for laboratory experiments. Any suggestions? Thanks!
10 August 2020 191 1 View
- I am testing the difference in carotenoids of 5 groups of diferent sex or development stage animals with one way ANOVA. My samples are from 13 the smallest to 44 the greatest. Do I use the eta...
11 April 2020 8,648 4 View
Hello, I have looked at your article "A novel functional calibration method for real-time elbow joint angles estimation with magnetic-inertial sensors" and was hoping for some additional...
04 March 2020 5,299 1 View
15 April 2019 1,244 6 View
I have a list of times (in hours) in an excel and i need to create entities at that specific times. You can find attached the times list. I have already created a file in Arena with those...
07 April 2019 9,848 3 View
Dear all, Recently I am performing RT-PCRs and Western blots of a mammalian cell line in which I overexpressed two vectors (wild-type and mutated) of equal promoter and sequence length. However,...
11 October 2018 6,037 5 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...
01 March 2021 6,142 3 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...
27 February 2021 9,176 3 View
Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...
27 February 2021 9,233 3 View
I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...
26 February 2021 10,041 3 View
I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...
25 February 2021 8,140 3 View
I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...
25 February 2021 6,635 5 View