Some ideas about contamination from Ca 2p always appearing and, therefore, overlaping with Pd3d in a SPECS spectrometer?

Dear colleagues, In our XPS instrument, most of the time when we acquire the Pd3d region (for a wide range of materials containing Pd) it overlaps with a strong signal from Ca2p. Nonetheless,...

10 November 2020 4,487 4 View

Working with weka for classification. Is it possible to classify ignoring (NO removing) a field/feautre?

Hi all, I am working with weka (software for machine learning). I have two sets of data, TRAIN and TEST. 1. The easiest question (I think). Is it possible to train modeland apply it to the TEST...

05 November 2020 3,317 3 View

Relation between social media habits and risk aversion?

Hi everyone, Im studying the relation between social media habits of customers and their risk aversion in order to create a model of machine learning to know which investment product fits better...

12 October 2020 5,975 3 View

Is it possible to increase the work performance of people in the HR industry through Emotional Intelligence training?

The question I'm asking is to clarify if there is a link between Emotional Intelligence and the HR Industry if there is a link, can we increase the work performance of people in the said industry...

17 August 2020 1,797 12 View

Where can I purchase lab -reared codling moth and Oriental fruit moth in the USA?

I need to purchase both moth species for laboratory experiments. Any suggestions? Thanks!

10 August 2020 191 1 View

How do you interpret the Cohen's d when you do a one way ANOVA. And how do you calculate it it? How do you report it?

- I am testing the difference in carotenoids of 5 groups of diferent sex or development stage animals with one way ANOVA. My samples are from 13 the smallest to 44 the greatest. Do I use the eta...

11 April 2020 8,648 4 View

How did you actually apply the functional calibration method from the OMC to the YEI sensors?

Hello, I have looked at your article "A novel functional calibration method for real-time elbow joint angles estimation with magnetic-inertial sensors" and was hoping for some additional...

04 March 2020 5,299 1 View

Does anybody know the name of this plant from the Canary Islands?

15 April 2019 1,244 6 View

In ARENA Simulation, how can i make entities arrive at a specific time?

I have a list of times (in hours) in an excel and i need to create entities at that specific times. You can find attached the times list. I have already created a file in Arena with those...

07 April 2019 9,848 3 View

Is it common find different levels of expression using almost equal overexpression vectors?

Dear all, Recently I am performing RT-PCRs and Western blots of a mammalian cell line in which I overexpressed two vectors (wild-type and mutated) of equal promoter and sequence length. However,...

11 October 2018 6,037 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View