And afther to read the expression this same labeled RNA can be quiantified by densitometry in a gel?
Hi everyone It is my first time working with this technology, so someone here can give me your experience my objective is to study different nucleolar lipids and their interaction with...
25 June 2024 7,351 0 View
Dear all, In specialized literature, it is usually reported that asymmetry due electron-hole pair creation becomes more significant for transition metals when the cluster size decreases. However,...
13 May 2024 4,428 2 View
Hi everyone, I hope someone could help me. I have two bacterial strains that I isolated by streaking them in solid media and collecting an individual colony, repeating this process 2-3 times to...
01 April 2024 6,866 3 View
I have 2D Resistivity Data. Can anyone explain that how to get 1D Data from 2D resistivity.
28 March 2024 8,278 0 View
I would like to use them as controls. I am studying the dynamics of receptors on the cell surface using SMI TIRF microscopy. I am currently using CHO cells and I would like to use constructs as...
19 December 2023 3,243 0 View
Please take 20 minutes and help me with this survey which is part of a master degree project I am supervising. This survey aims to validate and identify the relationship of design attributes or...
07 November 2023 7,591 0 View
We are running a milk of lime/brine reactors facility. We area suffering signifficant gypsum scaling issues. I´m looking for reactors design that overcomes it.
17 October 2023 5,504 0 View
Does anyone know of a passive RFID chip that has a hysteretic switch between a rectifier and an oscillator?
06 September 2022 5,541 0 View
I am trying to understand the structures of modern passive NFC/RFID chip rectifiers. Any help would be appreciated.
14 February 2022 1,270 2 View
For a research project, we are tasked with investigating the photoacclimation of greenhouse crops under varying light conditions (i.e. light quality, intensity, and photoperiod). Besides measuring...
13 February 2022 5,438 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I recently came across an anatomy text by Carl Moller that was published in 1915 but it is in German or Dutch neither of which I can understand. I would like to know if there is an English...
10 August 2024 4,347 1 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...
05 August 2024 9,805 3 View