I am trying to study DNA protein interaction using an entire promoter sequence (~1000bp).
I'm curious whether has functions can be used in mesh generation aspect of an fea code
10 May 2023 3,163 1 View
I'm looking to develop my knowledge to get hired as a stress engineer for my first job onwards. It seems like reinventing the wheel sometimes to try and understand how the software works. Is it...
30 April 2023 6,796 7 View
I am writing a FEA solver (using MATLAB) for computing stresses and stresses in a 2D rectangular plate with an elliptic hole (rotated to an angle). I computed the global stiffness matrix using...
27 April 2023 1,375 2 View
Imaging the other kidney in unilateral WT can sometimes be challenging especially with nephrogenic rests presenting as small WT. However, we have seen a case recently with wedge-shaped signal...
09 August 2021 9,382 1 View
Please suggest free software or online tool other than R software.
05 June 2021 8,355 0 View
I am working on a project on fungus and have prepared a few fungal slides which are present in and around the place I work [IISER Thiruvananthapuram,kerala, India]. Slides 1 and 6 could be...
25 March 2021 5,195 6 View
I'm looking for some kind of study, maybe a survey, that looks at where researchers started their work in terms of ideas and where they ended before publishing it. This could be an idea that they...
07 March 2021 4,450 5 View
I am planning to express a murine protein X with a Myc tag in HEK293F cell line - purify the protein - check the purity of the protein - use that protein - X with Myc tag to aspirate mouse. Thus,...
12 February 2020 4,585 1 View
I want know about an algorithm or formula to find the asymptote from coordinates obtained from Machine Learning. Like the ML will always give points precise & closer to the Asymptote if I ran it,...
11 October 2019 1,168 2 View
Please suggest easy and user friendly software for the same.....
19 February 2019 285 2 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
We assume this to be true. We also assume that the vacuum bomb is the latest version of explosives with an explosive power of a few to ten kg of TNT equivalent. It has the unique characteristic of...
04 August 2024 4,534 1 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View