if the culture was previously grown in media without penstrep, will a sudden switch to penstrep media kill the cells?
In my opinion it should not create any problems to the cells because pen-strep is an antibiotic that selectively targets the cell wall of bacteria.
I am trying to identify bacteria on skin that can utilise lactic acid as carbon source. So i intend to swab skin and then grow bacteria on minimal media supplemented with lactic acid. My question...
11 December 2018 3,556 0 View
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09 October 2018 9,908 0 View
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03 April 2018 5,924 0 View
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11 December 2017 2,657 0 View
hi i am not sure if it is alright to analyse my data this way. So i prepared 3 samples of controls, 3 samples of condition at 1hr exposure, 3 samples of condition at 6hr of exposure. i collected...
07 August 2017 7,902 5 View
why when wanting to look at genes that respond at say 1hr (early response), 6hr(intermediate response), 16hr (late response) it is better to look at RNA expression rather than protein? has it got...
07 August 2017 4,746 2 View
why is it that people usually look at mrna expression rather than protein expression when conducting a time course gene expression analysis?
06 July 2017 9,514 0 View
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kindly reply me. Thanking you in advance.
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I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
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