Hi everyone
Can I ask whether MALDI can detect organism straight from the positive bottle without first growing the colony/colonies?
Can you provide me/ direct me to the papers or sources of this information please.. Would love to read more.. Thanks
Barnini, S., Ghelardi, E., Brucculeri, V., Morici, P., and Lupetti, A. (2015). Rapid and reliable identification of Gram-negative bacteria and Gram-positive cocci by deposition of bacteria harvested from blood cultures onto the MALDI-TOF plate. BMC microbiology 15, 124.
Carbonnelle, E., Mesquita, C., Bille, E., Day, N., Dauphin, B., Beretti, J.-L., Ferroni, A., Gutmann, L., and Nassif, X. (2011). MALDI-TOF mass spectrometry tools for bacterial identification in clinical microbiology laboratory. Clin Biochem 44, 104-109.
Deak, E., Charlton, C.L., Bobenchik, A.M., Miller, S.A., Pollett, S., McHardy, I.H., Wu, M.T., and Garner, O.B. (2015). Comparison of the Vitek MS and Bruker Microflex LT MALDI-TOF MS platforms for routine identification of commonly isolated bacteria and yeast in the clinical microbiology laboratory. Diagn Microbiol Infect Dis 81, 27-33.
Kohlmann, R., Hoffmann, A., Geis, G., and Gatermann, S. (2015). MALDI-TOF mass spectrometry following short incubation on a solid medium is a valuable tool for rapid pathogen identification from positive blood cultures. Int J Med Microbiol 305, 469-479.
Schneiderhan, W., Grundt, A., Wörner, S., Findeisen, P., and Neumaier, M. (2013). Work flow analysis of around-the-clock processing of blood culture samples and integrated MALDI-TOF mass spectrometry analysis for the diagnosis of bloodstream infections. Clin Chem 59, 1649-1656.
Singhal, N., Kumar, M., Kanaujia, P.K., and Virdi, J.S. (2015). MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis. Front Microbiol 6, 791.
Hi Helmi Sulaiman,
if you are working with a Bruker Maldi-TOF system you might be interested in the new approved IVD test kits for direct pathogen identification from positive blood culture systems. See the following links...
http://ir.bruker.com/investors/press-releases/press-release-details/2015/Bruker-Introduces-CE-marked-IVD-MALDI-Sepsityper-Solution-for-Rapid-Identification-of-over-2200-Microorganisms-Directly-from-Positive-Blood-Culture/default.aspx
and
https://www.bruker.com/fileadmin/user_upload/8-PDF-Docs/Separations_MassSpectrometry/Literature/Brochures/1836106_MBT_Sepsityper_brochure_04-2015_eBook.pdf
In the flyer you will find additional scientic references.
Kind regards Wolf
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