For promoter assay I have chopped my promoter into six different segments and cloned each one into pGL3 basic vector for luciferase assay to analyse the activity of each segment. I co-transfect both firefly( which has the largest segment) and renilla vector into my experimental cell line into different ratios but both the values have been shown such low ones.In different journals, the RLU value generally shows 10,000 to 10,00000, but my experimental RLU values are very low, even in case of increased the amount of the vectors. Which is important- the RLU value or the normalized fold change activity? Please find the attached file where I have mentioned the RLU values.