How we can select ecotoxicological test organisms or test systems to evaluate residual toxicity of organic micropollutants in WWTP effluent?

Could you please introduce suitable references and reviews about the methods(any systems or organisms) for evaluation of the toxicity of organic micropollutants in WWTP effluent? and short summary...

22 September 2020 1,671 5 View

Will fixing and staining of the cells before diffusion assay affect the results so much?

Hi all, I am going to do a diffusion assay using FITC. My previous results were not illustrative. So, I think if I could stain it with DAPI first it would be more illustrative but I was wondering...

07 September 2020 3,063 3 View

Need suggestion for theoretical framework?

I'm doing a research on social media protest through thematic qualitative data analysis. But there is no traditional theory that can fit with my study. How do I arrenge this theoretical framework...

18 July 2020 7,928 2 View

Does somebody suggests some journals for social media research based on Asian continent?

I'm willing to submit my research work on a Bangladeshi social movement related to social media. It is a descriptive research based on a case study. But I'm in a confusion about choosing a...

26 November 2019 5,577 3 View

What is the best method to identify microalgae species in a mixture?

I am looking for a short and efficient method to identify microalgae species in a mixture of microalgae (and bacteria) live in wastewater.

23 January 2018 7,356 3 View

Does anybody know effective dose of GABA in diabetes in rats?

26 June 2016 3,626 4 View

Is there any brief scale for measuring Physical Activity in Diabetic patients?

i want to assess the physical activity of the diabetics and its association with diabetes control and quality of life along with other variables.

01 January 1970 3,701 8 View

Does theoretical framework essential for media/communication research?

Some high ranked journals are asking for theoretical framework regarding my social media research. Is it essential? Does anyone provide me some social media and communication journals with high...

01 January 1970 6,387 13 View

What chemicals should be used as a reactive diluent for alkyd?

01 January 1970 3,980 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View